Interaction of PC4 with melted DNA inhibits transcription
Identifieur interne : 003B53 ( Main/Exploration ); précédent : 003B52; suivant : 003B54Interaction of PC4 with melted DNA inhibits transcription
Auteurs : Sebastiaan Werten [Pays-Bas] ; Gertraud Stelzer [Allemagne] ; Andreas Goppelt [Allemagne] ; Friso M. Langen [Pays-Bas] ; Piet Gros [Pays-Bas] ; H. T. M. Timmers [Pays-Bas] ; Peter C. Van Der Vliet [Pays-Bas] ; Michael Meisterernst [Pays-Bas, Allemagne]Source :
- The EMBO Journal [ 0261-4189 ] ; 1998-09-01.
English descriptors
- Teeft :
- Activator, Antiparallel, Antiparallel strands, Basal transcription, Biol, Bubble oligonucleotide, Bubble templates, Ckii, Cofactor, Dsdna, Embo, Gene transcription, General factors, Gtfs, Gure legends, Heteroduplex, High concentrations, Higher concentrations, Initiation site, Kretzschmar, Late promoter, Mammalian cells, Minimal systems, Mutant, Mutation, Natl acad, Oligonucleotide, Open promoters, Phosphorylated, Phosphorylation, Polymerase, Premelted templates, Promoter, Recombinant, Represses, Represses transcription, Repression, Roeder, Ssdna, Template, Tfiib, Tfiid, Tfiie, Tfiih, Transcription, Transcription reactions, Transcriptional, Transcriptional activation, Transcriptional repression, Werten, Yeast.
Abstract
PC4 is a nuclear DNA‐binding protein that stimulates activator‐dependent class II gene transcription in vitro. Recent biochemical and X‐ray analyses have revealed a unique structure within the C‐terminal domain of PC4 that binds tightly to unpaired double‐stranded (ds)DNA. The cellular function of this evolutionarily conserved dimeric DNA‐binding fold is unknown. Here we demonstrate that PC4 represses transcription through this motif. Interaction with melted promoters is not required for activator‐dependent transcription in vitro. The inhibitory activity is attenuated on bona fide promoters by (i) transcription factor TFIIH and (ii) phosphorylation of PC4. PC4 remains a potent inhibitor of transcription in regions containing unpaired ds DNA, in single‐stranded DNA that can fold into two antiparallel strands, and on DNA ends. Our observations are consistent with a novel inhibitory function of PC4.
Url:
DOI: 10.1093/emboj/17.17.5103
Affiliations:
- Allemagne, Pays-Bas
- Bavière, District de Haute-Bavière, Utrecht (province)
- Munich, Utrecht
- Université Louis-et-Maximilien de Munich, Université d'Utrecht
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Le document en format XML
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Langen</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratorium voor Fysiologische Chemie, Utrecht University, Stratenum Universiteitsweg 100, CG, 3584, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Gros, Piet" sort="Gros, Piet" uniqKey="Gros P" first="Piet" last="Gros">Piet Gros</name><affiliation wicri:level="4"><orgName type="university">Université d'Utrecht</orgName><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Timmers, H T M" sort="Timmers, H T M" uniqKey="Timmers H" first="H. T. M." last="Timmers">H. T. M. Timmers</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratorium voor Fysiologische Chemie, Utrecht University, Stratenum Universiteitsweg 100, CG, 3584, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Van Der Vliet, Peter C" sort="Van Der Vliet, Peter C" uniqKey="Van Der Vliet P" first="Peter C." last="Van Der Vliet">Peter C. Van Der Vliet</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratorium voor Fysiologische Chemie, Utrecht University, Stratenum Universiteitsweg 100, CG, 3584, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Meisterernst, Michael" sort="Meisterernst, Michael" uniqKey="Meisterernst M" first="Michael" last="Meisterernst">Michael Meisterernst</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratorium voor Fysiologische Chemie, Utrecht University, Stratenum Universiteitsweg 100, CG, 3584, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The EMBO Journal</title><title level="j" type="alt">THE EMBO JOURNAL</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><biblScope unit="vol">17</biblScope><biblScope unit="issue">17</biblScope><biblScope unit="page" from="5103">5103</biblScope><biblScope unit="page" to="5111">5111</biblScope><biblScope unit="page-count">9</biblScope><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="1998-09-01">1998-09-01</date></imprint><idno type="ISSN">0261-4189</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0261-4189</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Activator</term><term>Antiparallel</term><term>Antiparallel strands</term><term>Basal transcription</term><term>Biol</term><term>Bubble oligonucleotide</term><term>Bubble templates</term><term>Ckii</term><term>Cofactor</term><term>Dsdna</term><term>Embo</term><term>Gene transcription</term><term>General factors</term><term>Gtfs</term><term>Gure legends</term><term>Heteroduplex</term><term>High concentrations</term><term>Higher concentrations</term><term>Initiation site</term><term>Kretzschmar</term><term>Late promoter</term><term>Mammalian cells</term><term>Minimal systems</term><term>Mutant</term><term>Mutation</term><term>Natl acad</term><term>Oligonucleotide</term><term>Open promoters</term><term>Phosphorylated</term><term>Phosphorylation</term><term>Polymerase</term><term>Premelted templates</term><term>Promoter</term><term>Recombinant</term><term>Represses</term><term>Represses transcription</term><term>Repression</term><term>Roeder</term><term>Ssdna</term><term>Template</term><term>Tfiib</term><term>Tfiid</term><term>Tfiie</term><term>Tfiih</term><term>Transcription</term><term>Transcription reactions</term><term>Transcriptional</term><term>Transcriptional activation</term><term>Transcriptional repression</term><term>Werten</term><term>Yeast</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">PC4 is a nuclear DNA‐binding protein that stimulates activator‐dependent class II gene transcription in vitro. Recent biochemical and X‐ray analyses have revealed a unique structure within the C‐terminal domain of PC4 that binds tightly to unpaired double‐stranded (ds)DNA. The cellular function of this evolutionarily conserved dimeric DNA‐binding fold is unknown. Here we demonstrate that PC4 represses transcription through this motif. Interaction with melted promoters is not required for activator‐dependent transcription in vitro. The inhibitory activity is attenuated on bona fide promoters by (i) transcription factor TFIIH and (ii) phosphorylation of PC4. PC4 remains a potent inhibitor of transcription in regions containing unpaired ds DNA, in single‐stranded DNA that can fold into two antiparallel strands, and on DNA ends. Our observations are consistent with a novel inhibitory function of PC4.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li><li>Utrecht (province)</li></region><settlement><li>Munich</li><li>Utrecht</li></settlement><orgName><li>Université Louis-et-Maximilien de Munich</li><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Werten, Sebastiaan" sort="Werten, Sebastiaan" uniqKey="Werten S" first="Sebastiaan" last="Werten">Sebastiaan Werten</name></region><name sortKey="Gros, Piet" sort="Gros, Piet" uniqKey="Gros P" first="Piet" last="Gros">Piet Gros</name><name sortKey="Langen, Friso M" sort="Langen, Friso M" uniqKey="Langen F" first="Friso M." last="Langen">Friso M. Langen</name><name sortKey="Meisterernst, Michael" sort="Meisterernst, Michael" uniqKey="Meisterernst M" first="Michael" last="Meisterernst">Michael Meisterernst</name><name sortKey="Timmers, H T M" sort="Timmers, H T M" uniqKey="Timmers H" first="H. T. M." last="Timmers">H. T. M. Timmers</name><name sortKey="Van Der Vliet, Peter C" sort="Van Der Vliet, Peter C" uniqKey="Van Der Vliet P" first="Peter C." last="Van Der Vliet">Peter C. Van Der Vliet</name></country><country name="Allemagne"><region name="Bavière"><name sortKey="Stelzer, Gertraud" sort="Stelzer, Gertraud" uniqKey="Stelzer G" first="Gertraud" last="Stelzer">Gertraud Stelzer</name></region><name sortKey="Goppelt, Andreas" sort="Goppelt, Andreas" uniqKey="Goppelt A" first="Andreas" last="Goppelt">Andreas Goppelt</name><name sortKey="Meisterernst, Michael" sort="Meisterernst, Michael" uniqKey="Meisterernst M" first="Michael" last="Meisterernst">Michael Meisterernst</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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